Coding

Part:BBa_K3733010:Design

Designed by: Zhenhao Han   Group: iGEM21_HZAU-China   (2021-09-28)


HepT: A toxin can cleave mRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We optimized the codon of HepT based on Escherichia coli codon bias.


Source

This part found through PubMed https://pubmed.ncbi.nlm.nih.gov/33342606/ and the sequence is synthesised by Genscript.

References

Wang X, Yao J, Sun Y C, et al. Type VII toxin/antitoxin classification system for antitoxins that enzymatically neutralize toxins[J]. Trends in Microbiology, 2020.